AustralieFrV1, Main, Exploration, bibRecord, 006545

acDCs enhance human antigen-specific T-cell responses

Identifieur interne : 006545 ( Main/Exploration ); précédent : 006544; suivant : 006546

acDCs enhance human antigen-specific T-cell responses

Auteurs : Emanuela Martinuzzi [France] ; Georgia Afonso [France] ; Marie-Claude Gagnerault [France] ; Gaetano Naselli [France] ; Diana Mittag [Australie] ; Béhazine Combadiere [France] ; Christian Boitard [France] ; Nathalie Chaput [France] ; Laurence Zitvogel [France] ; Leonard C. Harrison [Australie] ; Roberto Mallone [France]

Source :

Blood [ 0006-4971 ] ; 2011.

RBID : Pascal:11-0412241

Descripteurs français

Pascal (Inist)
- Homme, Réponse immune, Hématologie, Spécificité antigénique.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Antigen specific, Hematology, Human, Immune response.

Abstract

Detection of human Ag-specific T cells is limited by sensitivity and blood requirements. As dendritic cells (DCs) can potently stimulate T cells, we hypothesized that their induction in PBMCs in situ could link Ag processing and presentation to Ag-specific T-cell activation. To this end, unfractionated PBMCs (fresh or frozen) or whole blood were incubated for 48 hours with protein or peptide Ag together with different DC-activating agents to rapidly and sequentially induce, pulse, and mature DCs. DC activation was therefore lined up with Ag recognition by neighboring T cells, thus telescoping the sequential steps of T-cell activation. Efficient processing of protein Ags made prior knowledge of epitopes and HLA restrictions dispensable. While reducing stimulation time, manipulation and blood requirements, in situ DC induction specifically amplified Ag-specific T-cell responses (cytokine secretion, proliferation, CD137/CD154 up-regulation, and binding of peptide-HLA multimers). IL-1β, although released by DCs, was also secreted in an Ag-specific fashion, thus providing an indirect biomarker of T-cell responses. These accelerated cocultured DC (acDC) assays offered a sensitive means with which to evaluate T-cell responses to viral and melanoma Ag vaccination, and may therefore find application for immune monitoring in viral, tumor, autoimmune, and transplantation settings.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 001953
to stream PascalFrancis, to step Curation: 004602
to stream PascalFrancis, to step Checkpoint: 001471
to stream Main, to step Merge: 006922
to stream Main, to step Curation: 006545

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">acDCs enhance human antigen-specific T-cell responses</title>
<author><name sortKey="Martinuzzi, Emanuela" sort="Martinuzzi, Emanuela" uniqKey="Martinuzzi E" first="Emanuela" last="Martinuzzi">Emanuela Martinuzzi</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Afonso, Georgia" sort="Afonso, Georgia" uniqKey="Afonso G" first="Georgia" last="Afonso">Georgia Afonso</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Gagnerault, Marie Claude" sort="Gagnerault, Marie Claude" uniqKey="Gagnerault M" first="Marie-Claude" last="Gagnerault">Marie-Claude Gagnerault</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Naselli, Gaetano" sort="Naselli, Gaetano" uniqKey="Naselli G" first="Gaetano" last="Naselli">Gaetano Naselli</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Mittag, Diana" sort="Mittag, Diana" uniqKey="Mittag D" first="Diana" last="Mittag">Diana Mittag</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The Walter and Eliza Hall Institute of Medical Research, Parkville</s1>
<s2>Victoria</s2>
<s3>AUS</s3>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Combadiere, Behazine" sort="Combadiere, Behazine" uniqKey="Combadiere B" first="Béhazine" last="Combadiere">Béhazine Combadiere</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Inserm U945</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Université Pierre et Marie Curie, Laboratory of Immunity and Infection</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Pierre-et-Marie-Curie</orgName>
</affiliation>
</author>
<author><name sortKey="Boitard, Christian" sort="Boitard, Christian" uniqKey="Boitard C" first="Christian" last="Boitard">Christian Boitard</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Chaput, Nathalie" sort="Chaput, Nathalie" uniqKey="Chaput N" first="Nathalie" last="Chaput">Nathalie Chaput</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Inserm U1015, Institut Gustave Roussy</s1>
<s2>Villejuif</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>Villejuif</wicri:noRegion>
<wicri:noRegion>Institut Gustave Roussy</wicri:noRegion>
<wicri:noRegion>Villejuif</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Centre d'Investigation Clinique Biothérapie 507</s1>
<s2>Villejuif</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>Villejuif</wicri:noRegion>
<wicri:noRegion>Centre d'Investigation Clinique Biothérapie 507</wicri:noRegion>
<wicri:noRegion>Centre d'Investigation Clinique Biothérapie 507</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zitvogel, Laurence" sort="Zitvogel, Laurence" uniqKey="Zitvogel L" first="Laurence" last="Zitvogel">Laurence Zitvogel</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Inserm U1015, Institut Gustave Roussy</s1>
<s2>Villejuif</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>Villejuif</wicri:noRegion>
<wicri:noRegion>Institut Gustave Roussy</wicri:noRegion>
<wicri:noRegion>Villejuif</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Centre d'Investigation Clinique Biothérapie 507</s1>
<s2>Villejuif</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>Villejuif</wicri:noRegion>
<wicri:noRegion>Centre d'Investigation Clinique Biothérapie 507</wicri:noRegion>
<wicri:noRegion>Centre d'Investigation Clinique Biothérapie 507</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="08"><s1>Université de Médecine Kremlin Bicêtre</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Harrison, Leonard C" sort="Harrison, Leonard C" uniqKey="Harrison L" first="Leonard C." last="Harrison">Leonard C. Harrison</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The Walter and Eliza Hall Institute of Medical Research, Parkville</s1>
<s2>Victoria</s2>
<s3>AUS</s3>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mallone, Roberto" sort="Mallone, Roberto" uniqKey="Mallone R" first="Roberto" last="Mallone">Roberto Mallone</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">11-0412241</idno>
<date when="2011">2011</date>
<idno type="stanalyst">PASCAL 11-0412241 INIST</idno>
<idno type="RBID">Pascal:11-0412241</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">001953</idno>
<idno type="wicri:Area/PascalFrancis/Curation">004602</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">001471</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">001471</idno>
<idno type="wicri:doubleKey">0006-4971:2011:Martinuzzi E:acdcs:enhance:human</idno>
<idno type="wicri:Area/Main/Merge">006922</idno>
<idno type="wicri:Area/Main/Curation">006545</idno>
<idno type="wicri:Area/Main/Exploration">006545</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">acDCs enhance human antigen-specific T-cell responses</title>
<author><name sortKey="Martinuzzi, Emanuela" sort="Martinuzzi, Emanuela" uniqKey="Martinuzzi E" first="Emanuela" last="Martinuzzi">Emanuela Martinuzzi</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Afonso, Georgia" sort="Afonso, Georgia" uniqKey="Afonso G" first="Georgia" last="Afonso">Georgia Afonso</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Gagnerault, Marie Claude" sort="Gagnerault, Marie Claude" uniqKey="Gagnerault M" first="Marie-Claude" last="Gagnerault">Marie-Claude Gagnerault</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Naselli, Gaetano" sort="Naselli, Gaetano" uniqKey="Naselli G" first="Gaetano" last="Naselli">Gaetano Naselli</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Mittag, Diana" sort="Mittag, Diana" uniqKey="Mittag D" first="Diana" last="Mittag">Diana Mittag</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The Walter and Eliza Hall Institute of Medical Research, Parkville</s1>
<s2>Victoria</s2>
<s3>AUS</s3>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Combadiere, Behazine" sort="Combadiere, Behazine" uniqKey="Combadiere B" first="Béhazine" last="Combadiere">Béhazine Combadiere</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Inserm U945</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Université Pierre et Marie Curie, Laboratory of Immunity and Infection</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Pierre-et-Marie-Curie</orgName>
</affiliation>
</author>
<author><name sortKey="Boitard, Christian" sort="Boitard, Christian" uniqKey="Boitard C" first="Christian" last="Boitard">Christian Boitard</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
<author><name sortKey="Chaput, Nathalie" sort="Chaput, Nathalie" uniqKey="Chaput N" first="Nathalie" last="Chaput">Nathalie Chaput</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Inserm U1015, Institut Gustave Roussy</s1>
<s2>Villejuif</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>Villejuif</wicri:noRegion>
<wicri:noRegion>Institut Gustave Roussy</wicri:noRegion>
<wicri:noRegion>Villejuif</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Centre d'Investigation Clinique Biothérapie 507</s1>
<s2>Villejuif</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>Villejuif</wicri:noRegion>
<wicri:noRegion>Centre d'Investigation Clinique Biothérapie 507</wicri:noRegion>
<wicri:noRegion>Centre d'Investigation Clinique Biothérapie 507</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zitvogel, Laurence" sort="Zitvogel, Laurence" uniqKey="Zitvogel L" first="Laurence" last="Zitvogel">Laurence Zitvogel</name>
<affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Inserm U1015, Institut Gustave Roussy</s1>
<s2>Villejuif</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>Villejuif</wicri:noRegion>
<wicri:noRegion>Institut Gustave Roussy</wicri:noRegion>
<wicri:noRegion>Villejuif</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Centre d'Investigation Clinique Biothérapie 507</s1>
<s2>Villejuif</s2>
<s3>FRA</s3>
<sZ>8 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<wicri:noRegion>Villejuif</wicri:noRegion>
<wicri:noRegion>Centre d'Investigation Clinique Biothérapie 507</wicri:noRegion>
<wicri:noRegion>Centre d'Investigation Clinique Biothérapie 507</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="08"><s1>Université de Médecine Kremlin Bicêtre</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Harrison, Leonard C" sort="Harrison, Leonard C" uniqKey="Harrison L" first="Leonard C." last="Harrison">Leonard C. Harrison</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>The Walter and Eliza Hall Institute of Medical Research, Parkville</s1>
<s2>Victoria</s2>
<s3>AUS</s3>
<sZ>5 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mallone, Roberto" sort="Mallone, Roberto" uniqKey="Mallone R" first="Roberto" last="Mallone">Roberto Mallone</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Inserm U986, Diabetes & Autoimmunity Research Laboratory</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Université Paris Descartes, Faculté de Médecine Rene Descartes</s1>
<s2>Paris</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Paris-Descartes</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Blood</title>
<title level="j" type="abbreviated">Blood</title>
<idno type="ISSN">0006-4971</idno>
<imprint><date when="2011">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Blood</title>
<title level="j" type="abbreviated">Blood</title>
<idno type="ISSN">0006-4971</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigen specific</term>
<term>Hematology</term>
<term>Human</term>
<term>Immune response</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Homme</term>
<term>Réponse immune</term>
<term>Hématologie</term>
<term>Spécificité antigénique</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Detection of human Ag-specific T cells is limited by sensitivity and blood requirements. As dendritic cells (DCs) can potently stimulate T cells, we hypothesized that their induction in PBMCs in situ could link Ag processing and presentation to Ag-specific T-cell activation. To this end, unfractionated PBMCs (fresh or frozen) or whole blood were incubated for 48 hours with protein or peptide Ag together with different DC-activating agents to rapidly and sequentially induce, pulse, and mature DCs. DC activation was therefore lined up with Ag recognition by neighboring T cells, thus telescoping the sequential steps of T-cell activation. Efficient processing of protein Ags made prior knowledge of epitopes and HLA restrictions dispensable. While reducing stimulation time, manipulation and blood requirements, in situ DC induction specifically amplified Ag-specific T-cell responses (cytokine secretion, proliferation, CD137/CD154 up-regulation, and binding of peptide-HLA multimers). IL-1β, although released by DCs, was also secreted in an Ag-specific fashion, thus providing an indirect biomarker of T-cell responses. These accelerated cocultured DC (acDC) assays offered a sensitive means with which to evaluate T-cell responses to viral and melanoma Ag vaccination, and may therefore find application for immune monitoring in viral, tumor, autoimmune, and transplantation settings.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
</country>
<region><li>Île-de-France</li>
</region>
<settlement><li>Paris</li>
</settlement>
<orgName><li>Université Paris-Descartes</li>
<li>Université Pierre-et-Marie-Curie</li>
</orgName>
</list>
<tree><country name="France"><region name="Île-de-France"><name sortKey="Martinuzzi, Emanuela" sort="Martinuzzi, Emanuela" uniqKey="Martinuzzi E" first="Emanuela" last="Martinuzzi">Emanuela Martinuzzi</name>
</region>
<name sortKey="Afonso, Georgia" sort="Afonso, Georgia" uniqKey="Afonso G" first="Georgia" last="Afonso">Georgia Afonso</name>
<name sortKey="Afonso, Georgia" sort="Afonso, Georgia" uniqKey="Afonso G" first="Georgia" last="Afonso">Georgia Afonso</name>
<name sortKey="Boitard, Christian" sort="Boitard, Christian" uniqKey="Boitard C" first="Christian" last="Boitard">Christian Boitard</name>
<name sortKey="Boitard, Christian" sort="Boitard, Christian" uniqKey="Boitard C" first="Christian" last="Boitard">Christian Boitard</name>
<name sortKey="Chaput, Nathalie" sort="Chaput, Nathalie" uniqKey="Chaput N" first="Nathalie" last="Chaput">Nathalie Chaput</name>
<name sortKey="Chaput, Nathalie" sort="Chaput, Nathalie" uniqKey="Chaput N" first="Nathalie" last="Chaput">Nathalie Chaput</name>
<name sortKey="Combadiere, Behazine" sort="Combadiere, Behazine" uniqKey="Combadiere B" first="Béhazine" last="Combadiere">Béhazine Combadiere</name>
<name sortKey="Combadiere, Behazine" sort="Combadiere, Behazine" uniqKey="Combadiere B" first="Béhazine" last="Combadiere">Béhazine Combadiere</name>
<name sortKey="Gagnerault, Marie Claude" sort="Gagnerault, Marie Claude" uniqKey="Gagnerault M" first="Marie-Claude" last="Gagnerault">Marie-Claude Gagnerault</name>
<name sortKey="Gagnerault, Marie Claude" sort="Gagnerault, Marie Claude" uniqKey="Gagnerault M" first="Marie-Claude" last="Gagnerault">Marie-Claude Gagnerault</name>
<name sortKey="Mallone, Roberto" sort="Mallone, Roberto" uniqKey="Mallone R" first="Roberto" last="Mallone">Roberto Mallone</name>
<name sortKey="Mallone, Roberto" sort="Mallone, Roberto" uniqKey="Mallone R" first="Roberto" last="Mallone">Roberto Mallone</name>
<name sortKey="Martinuzzi, Emanuela" sort="Martinuzzi, Emanuela" uniqKey="Martinuzzi E" first="Emanuela" last="Martinuzzi">Emanuela Martinuzzi</name>
<name sortKey="Naselli, Gaetano" sort="Naselli, Gaetano" uniqKey="Naselli G" first="Gaetano" last="Naselli">Gaetano Naselli</name>
<name sortKey="Naselli, Gaetano" sort="Naselli, Gaetano" uniqKey="Naselli G" first="Gaetano" last="Naselli">Gaetano Naselli</name>
<name sortKey="Zitvogel, Laurence" sort="Zitvogel, Laurence" uniqKey="Zitvogel L" first="Laurence" last="Zitvogel">Laurence Zitvogel</name>
<name sortKey="Zitvogel, Laurence" sort="Zitvogel, Laurence" uniqKey="Zitvogel L" first="Laurence" last="Zitvogel">Laurence Zitvogel</name>
<name sortKey="Zitvogel, Laurence" sort="Zitvogel, Laurence" uniqKey="Zitvogel L" first="Laurence" last="Zitvogel">Laurence Zitvogel</name>
</country>
<country name="Australie"><noRegion><name sortKey="Mittag, Diana" sort="Mittag, Diana" uniqKey="Mittag D" first="Diana" last="Mittag">Diana Mittag</name>
</noRegion>
<name sortKey="Harrison, Leonard C" sort="Harrison, Leonard C" uniqKey="Harrison L" first="Leonard C." last="Harrison">Leonard C. Harrison</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006545 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006545 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:11-0412241
   |texte=   acDCs enhance human antigen-specific T-cell responses
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les relations entre la France et l'Australie

acDCs enhance human antigen-specific T-cell responses

acDCs enhance human antigen-specific T-cell responses

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri